Deprecated: Use of Please install wikidiff2 to retain inline diff functionality. was deprecated in MediaWiki 1.35.0. [Called from SpecialMobileDiff::executeWhenAvailable in /volume3/web/w/extensions/MobileFrontend/includes/specials/SpecialMobileDiff.php at line 146] in /volume3/web/w/includes/debug/MWDebug.php on line 376

Call Stack:
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    0.0233    2606984   2. wfIndexMain() /volume3/web/w/index.php:46
    0.0233    2609128   3. MediaWiki->run() /volume3/web/w/index.php:53
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private $mHTMLtitle = 'Changes - Jesse\'s Wiki'; private $mIsArticle = FALSE; private $mIsArticleRelated = FALSE; private $mHasCopyright = FALSE; private $mPrintable = FALSE; private $mSubtitle = [...]; public $mRedirect = ''; protected $mStatusCode = NULL; protected $mLastModified = ''; protected $mCategoryLinks = [...]; protected $mCategories = [...]; protected $mIndicators = [...]; private $mLanguageLinks = [...]; private $mScripts = ''; protected $mInlineStyles = ''; public $mPageLinkTitle = ''; protected $mAdditionalHtmlClasses = [...]; protected $mHeadItems = [...]; protected $mAdditionalBodyClasses = [...]; protected $mModules = [...]; protected $mModuleStyles = [...]; protected $mResourceLoader = class ResourceLoader { ... }; private $rlClient = NULL; private $rlClientContext = NULL; private $rlExemptStyleModules = NULL; protected $mJsConfigVars = [...]; protected $mTemplateIds = [...]; protected $mImageTimeKeys = [...]; public $mRedirectCode = ''; protected $mFeedLinksAppendQuery = NULL; protected $mAllowedModules = [...]; protected $mDoNothing = FALSE; protected $mContainsNewMagic = 0; protected $mParserOptions = NULL; private $mFeedLinks = [...]; protected $mEnableClientCache = TRUE; private $mArticleBodyOnly = FALSE; protected $mNewSectionLink = FALSE; protected $mHideNewSectionLink = FALSE; public $mNoGallery = FALSE; protected $mCdnMaxage = 0; protected $mCdnMaxageLimit = INF; protected $mPreventClickjacking = TRUE; private $mRevisionId = NULL; private $mRevisionTimestamp = NULL; protected $mFileVersion = NULL; protected $styles = [...]; private $mIndexPolicy = 'noindex'; private $mFollowPolicy = 'nofollow'; private $mVaryHeader = [...]; private $mRedirectedFrom = NULL; private $mProperties = [...]; private $mTarget = NULL; private $mEnableTOC = FALSE; private $copyrightUrl = NULL; private $limitReportJSData = [...]; private $contentOverrides = [...]; private $contentOverrideCallbacks = [...]; private $mLinkHeader = [...]; private $CSP = class ContentSecurityPolicy { ... }; 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    0.0441    3415776   7. SpecialPage->run($subPage = '7') /volume3/web/w/includes/specialpage/SpecialPageFactory.php:1386
    0.0454    3491640   8. MobileSpecialPage->execute($subPage = '7') /volume3/web/w/includes/specialpage/SpecialPage.php:646
    0.0455    3492128   9. SpecialMobileDiff->executeWhenAvailable($par = '7') /volume3/web/w/extensions/MobileFrontend/includes/specials/MobileSpecialPage.php:71
    0.0680    4602720  10. SpecialMobileDiff->displayDiffPage() /volume3/web/w/extensions/MobileFrontend/includes/specials/SpecialMobileDiff.php:146
    0.0916    4805648  11. wfDeprecated($function = 'Please install wikidiff2 to retain inline diff functionality.', $version = '1.35.0', $component = ???, $callerOffset = ???) /volume3/web/w/extensions/MobileFrontend/includes/specials/SpecialMobileDiff.php:187
    0.0916    4805648  12. MWDebug::deprecated($function = 'Please install wikidiff2 to retain inline diff functionality.', $version = '1.35.0', $component = FALSE, $callerOffset = 3) /volume3/web/w/includes/GlobalFunctions.php:1036
    0.0916    4805808  13. MWDebug::deprecatedMsg($msg = 'Use of Please install wikidiff2 to retain inline diff functionality. was deprecated in MediaWiki 1.35.0.', $version = '1.35.0', $component = 'MediaWiki', $callerOffset = 4) /volume3/web/w/includes/debug/MWDebug.php:230
    0.0917    4807576  14. MWDebug::sendRawDeprecated($msg = 'Use of Please install wikidiff2 to retain inline diff functionality. was deprecated in MediaWiki 1.35.0. [Called from SpecialMobileDiff::executeWhenAvailable in /volume3/web/w/extensions/MobileFrontend/includes/specials/SpecialMobileDiff.php at line 146]', $sendToLog = TRUE, $callerFunc = 'SpecialMobileDiff::executeWhenAvailable') /volume3/web/w/includes/debug/MWDebug.php:350
    0.0917    4807576  15. trigger_error($message = 'Use of Please install wikidiff2 to retain inline diff functionality. was deprecated in MediaWiki 1.35.0. [Called from SpecialMobileDiff::executeWhenAvailable in /volume3/web/w/extensions/MobileFrontend/includes/specials/SpecialMobileDiff.php at line 146]', $error_type = 16384) /volume3/web/w/includes/debug/MWDebug.php:376


Warning: Cannot modify header information - headers already sent by (output started at /volume3/web/w/includes/debug/MWDebug.php:376) in /volume3/web/w/includes/WebResponse.php on line 74

Call Stack:
    0.0001     359600   1. {main}() /volume3/web/w/index.php:0
    0.0233    2606984   2. wfIndexMain() /volume3/web/w/index.php:46
    0.0233    2609128   3. MediaWiki->run() /volume3/web/w/index.php:53
    0.0237    2609384   4. MediaWiki->main() /volume3/web/w/includes/MediaWiki.php:546
    0.1273    5096784   5. MediaWiki::{closure:/volume3/web/w/includes/MediaWiki.php:925-931}() /volume3/web/w/includes/MediaWiki.php:940
    0.1273    5096784   6. OutputPage->output($return = TRUE) /volume3/web/w/includes/MediaWiki.php:927
    0.1274    5113360   7. WebResponse->header($string = 'Content-type: text/html; charset=UTF-8', $replace = ???, $http_response_code = ???) /volume3/web/w/includes/OutputPage.php:2583
    0.1275    5113584   8. header($header = 'Content-type: text/html; charset=UTF-8', $replace = TRUE) /volume3/web/w/includes/WebResponse.php:74


Warning: Cannot modify header information - headers already sent by (output started at /volume3/web/w/includes/debug/MWDebug.php:376) in /volume3/web/w/includes/WebResponse.php on line 74

Call Stack:
    0.0001     359600   1. {main}() /volume3/web/w/index.php:0
    0.0233    2606984   2. wfIndexMain() /volume3/web/w/index.php:46
    0.0233    2609128   3. MediaWiki->run() /volume3/web/w/index.php:53
    0.0237    2609384   4. MediaWiki->main() /volume3/web/w/includes/MediaWiki.php:546
    0.1273    5096784   5. MediaWiki::{closure:/volume3/web/w/includes/MediaWiki.php:925-931}() /volume3/web/w/includes/MediaWiki.php:940
    0.1273    5096784   6. OutputPage->output($return = TRUE) /volume3/web/w/includes/MediaWiki.php:927
    0.1279    5113568   7. WebResponse->header($string = 'Content-language: en', $replace = ???, $http_response_code = ???) /volume3/web/w/includes/OutputPage.php:2585
    0.1279    5113568   8. header($header = 'Content-language: en', $replace = TRUE) /volume3/web/w/includes/WebResponse.php:74


Warning: Cannot modify header information - headers already sent by (output started at /volume3/web/w/includes/debug/MWDebug.php:376) in /volume3/web/w/includes/WebResponse.php on line 74

Call Stack:
    0.0001     359600   1. {main}() /volume3/web/w/index.php:0
    0.0233    2606984   2. wfIndexMain() /volume3/web/w/index.php:46
    0.0233    2609128   3. MediaWiki->run() /volume3/web/w/index.php:53
    0.0237    2609384   4. MediaWiki->main() /volume3/web/w/includes/MediaWiki.php:546
    0.1273    5096784   5. MediaWiki::{closure:/volume3/web/w/includes/MediaWiki.php:925-931}() /volume3/web/w/includes/MediaWiki.php:940
    0.1273    5096784   6. OutputPage->output($return = TRUE) /volume3/web/w/includes/MediaWiki.php:927
    0.1284    5113568   7. WebResponse->header($string = 'X-Frame-Options: DENY', $replace = ???, $http_response_code = ???) /volume3/web/w/includes/OutputPage.php:2595
    0.1284    5113568   8. header($header = 'X-Frame-Options: DENY', $replace = TRUE) /volume3/web/w/includes/WebResponse.php:74

<!DOCTYPE html>
<html class="client-nojs" lang="en" dir="ltr">
<head>
<meta charset="UTF-8"/>
<title>Changes - Jesse's Wiki</title>
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<link rel="EditURI" type="application/rsd+xml" href="https://wiki.jjjp.ca/api.php?action=rsd"/>
<link rel="alternate" type="application/atom+xml" title="Jesse&#039;s Wiki Atom feed" href="/index.php?title=Special:RecentChanges&amp;feed=atom"/>
</head>
<body class="mediawiki ltr sitedir-ltr mw-hide-empty-elt ns--1 ns-special mw-special-MobileDiff page-Special_MobileDiff_7 rootpage-Special_MobileDiff_7 skin-vector action-view skin-vector-legacy"><div id="mw-page-base" class="noprint"></div>
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<div id="content" class="mw-body" role="main">
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	<div id="siteNotice" class="mw-body-content"></div>
	<div class="mw-indicators mw-body-content">
	</div>
	<h1 id="firstHeading" class="firstHeading" >Changes</h1>
	<div id="bodyContent" class="mw-body-content">
		
		<div id="contentSub"></div>
		<div id="contentSub2"></div>
		
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		<div id="mw-content-text"><div id="mw-mf-diffview" class="content-unstyled"><div id="mw-mf-diffarea"><div class="mw-mf-diff-info">
	<h2 class="mw-mf-diff-info__link-latest">
		<a href="/w/Formalin_Fixation">Formalin Fixation</a>
		<a href="/index.php?title=Formalin_Fixation&amp;action=edit">(view source)</a>
	</h2>
	<h3 class="mw-mf-diff-info__link-revision">
		<a href="/index.php?title=Formalin_Fixation&amp;oldid=7">Revision as of 01:00, 13 September 2020</a>
	</h3>
	<span class="mw-ui-icon mw-ui-icon-before mw-ui-icon-mf-upTriangle-constructive meta mw-ui-icon-small mw-mf-bytesadded">
		<span>919 bytes added</span>
	</span><span class="mw-mf-diff-date">
		, &nbsp;<span>01:00, 13 September 2020</span>
	</span>
</div>
<div class="mw-mf-diff-comment">init</div><div id="mw-mf-minidiff"><ins>* poor morphology<br />
** struggle to make diagnosis, if at all possible<br />
* IHC<br />
** primary diagnosis <br />
** prognostic / predictive factors<br />
*** ER, PR HER2/Neu<br />
*** Mismatch repair proteins<br />
*** PD-L1 and other biomarkers<br />
<br />
Fixation<br />
* stability of tissue components<br />
* increases avidity for dyes <br />
<br />
Poor Fixation<br />
* degradation/sloughing of mucosa layer (like in colon)<br />
* contour and detail<br />
<br />
Irreversible Fixation<br />
* covalent bonding by methylene bridges, altering structure<br />
* over-fixation makes this worse, irreversible altered protein shape<br />
* standardized IHC tests rely on a certain degree of how altered the protein shape gets<br />
* Newfoundland breast cancer scandal Cameron Inquiry https://www.cihrt.nl.ca/transcripts.html<br />
* particularly important for membranous pattern stains, like CD20 (as opposed to nuclear or cytoplasmic)<br />
<br />
Decalcification<br />
* destroys RNA, DNA<br />
* particularly important to note for metastases (Primary diagnosis)</ins></div><div class="patrollink"></div></div><div id="mw-mf-userinfo" class="position-fixed"><div class="post-content"><div class="mw-ui-icon mw-ui-icon-before mw-ui-icon-mf-userAvatar-base20 mw-mf-user" data-revision-id="7" data-user-name="Scripcat" data-user-gender="unknown"><a href="/index.php?title=User:Scripcat&amp;action=view" class="new mw-mf-user-link" title="User:Scripcat (page does not exist)">Scripcat</a></div><div class="mw-mf-roles meta"></div><div class="mw-mf-edit-count meta"><div>5</div><p> edits</p></div></div></div></div>
<div class="printfooter">Retrieved from "<a dir="ltr" href="https://wiki.jjjp.ca/w/Special:MobileDiff/7">https://wiki.jjjp.ca/w/Special:MobileDiff/7</a>"</div></div>
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</div>

<div id="mw-navigation">
	<h2>Navigation menu</h2>
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<nav id="p-personal" class="mw-portlet mw-portlet-personal vector-menu" aria-labelledby="p-personal-label" role="navigation" 
	 >
	<h3 id="p-personal-label" class="vector-menu-heading">
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